Samy Dufour, PhD

TITLE: Comprehensive Solutions for the Formulation and Spatial Characterization of 3D Immune Models with Thermo Fisher Three-dimensional (3D) cell culture systems, including organoids, spheroids, and tumoroids, are rapidly becoming widely used in immune modeling. By recapitulating tissue architecture, cellular heterogeneity, and diffusion-driven gradients, these models provide a physiologically relevant framework to investigate complex mechanisms such as immune cell infiltration, activation, and therapeutic response. However, standardizing both the generation and analysis of these complex microenvironments remains a key challenge. Once established, extracting meaningful biological insights requires robust and spatially resolved characterization. Advanced imaging plays a central role by enabling both structural and functional analysis of complex 3D models. Brightfield imaging allows rapid monitoring of spheroid size and morphology, while fluorescence-based approaches enable multiplexed analysis of immune and cellular markers. Thermo Fisher Scientific streamlines these workflows by combining high-performance imaging platforms with validated reagents, following a philosophy of user-friendly systems. Automated systems (e.g., Invitrogen™ EVOS™ M7000 Imaging System) enable fast, reproducible imaging for routine monitoring and live cell analysis. High-content confocal screening platforms (e.g., Thermo Scientific™ CellInsight™ CX7 LZR Pro High Content Screening Platform) enable automated, multiparametric imaging of thick 3D samples. Finally, spatial imaging solutions (e.g., EVOS™ S1000 Spatial Imaging System) enable up to 9-color imaging for fast detailed tissue mapping.