Gintarė Gervaitytė

Rolling Circle Amplification (RCA) is a powerful molecular biology technique that generates long single-stranded DNA molecules from circular templates. RCA reactions are performed under isothermal conditions and use strand-displacing polymerases, such as phi29 DNA polymerase.

Due to its high efficiency and sensitivity, RCA is perfect for applications like SNP detection, next-generation sequencing, in vitro transcription, cell-free DNA enrichment, and cell-free protein expression.

The session will cover the fundamental principles of RCA, its diverse applications, and practical examples. Let‘s discover how RCA can advance your molecular biology research!