TITLE: Exploring the Possibilities of Loop-Mediated Isothermal Amplification

In the face of the rising prevalence of infectious diseases, the need for rapid testing has never been more important. Technologies like Loop-mediated isothermal amplification can be a great solution for the fast and simple detection of infectious diseases. LAMP is a method used for DNA amplification under isothermal conditions that utilizes a DNA polymerase with strong strand displacement activity, such as Bst DNA polymerase. Because of the method‘s simplicity and rapidity, it has become a valuable technology for on-the-spot diagnostics and can be adapted to create molecular tests suitable for point-of-care applications.

TITLE: Pipetting Best Practices

Of all the factors that contribute to the performance of a pipette, the most critical ones are the skill & expertise of the operator. Are you pipetting different liquids? Are you interested in improving reproducibility of your assays? With this Pipetting Best Practice seminar we will go through the different pipetting techniques and guide you through the recommendations of how to pipette different liquids. There are several factors that can affect the accuracy of pipetting and we will give you quick tips and tricks to achieve better accuracy and how to prevent cross contamination. If you feel pain in your thumb and wrist while pipetting, Thermo Fisher Scientific offers a lot of ergonomics tools to minimize repetitive strain injuries. If you want to learn more about pipetting, please join us at this session and visit our booth.

TITLE: PCR Clean Up, Size Selection and Green Dynabeads

Do you want to do DNA/RNA/Protein and cell extraction with only one instrument? You want to have an open platform? Kit independent?  With heating and cooling? Easy to use software for all applications? To know more about PCR Clean up and size selection and green Dynabeads. If so or only some parts of it, we have the solution for it. The new KingFisher APEX DX gives all these applications with only one instrument, one software and a variety of kits for your choice. You can control each step of the extraction process by software and fine tune it to your needs. If you want to know more about so join us in the lunch session and visit our booth.

TITLE: Biological Safety Cabinets – Focus on Safety

When on the search for a new Biological Safety Cabinet it can be difficult to know what to look for and why. This presentation will focus on good practices when working in a Biological Safety Cabinet and what goes on behind the scenes in a cabinet, the HEPA filters, the airflows, and the sensor systems that keeps both you and your samples safe. A section will be dedicated to the EN12469 and the tests that must be preformed to ensure the cabinet is functional from day 1.

TITLE: Sample Protection: Optimizing the Layout, Capacity, and Design of  Your Cold Storage

Let’s discuss safe sample protection during cold storage, looking at how to optimize the preservation of your samples, in terms of equipment properties, sample containment, support systems, plus other key external factors.

Your cold storage equipment, whether it is a laboratory refrigerator or freezer, ULT or LN2 cryogenic freezer, should be considered a safety device in place to protect what’s stored in it. Perfect sample storage is not just determined by the storage unit itself but also by a multitude of other components, external factors and processes.

Let’s talk about Optimizing the layout, capacity, and design of your cold storage equipment, the role of support systems in keeping your sample safe, how maintenance, timely care, lab layout and organization contribute to sample protection and important performance criteria, such as cost of ownership, ergonomics, user safety and sustainability.

TITLE: Evolution of Phusion DNA Polymerases

PCR has revolutionized modern molecular biology, but its success would not be possible without the advancements in DNA polymerases. The initial polymerase used in PCR reactions was thermolabile, requiring replenishment in every cycle, making it a slow and monotonous process. Fortunately, modern thermostable and fast polymerases now offer accurate results, optimal performance, and streamlined workflows. Gain insight into the evolution of Phusion DNA polymerase technology and learn how this enzyme was improved during the last 20 years to make it the most suitable polymerase for various high fidelity and challenging PCR applications.

TITLE: Flow Cytometry as You‘ve Never Seen it

The Invitrogen Attune CytPix Flow Cytometer integrates acoustic focusing and a high-speed brightfield camera for simultaneous high-throughput flow cytometry and detailed brightfield imaging. Automated image analysis converts event features into extended parameters, enhancing standard flow data. Flow cytometry faces challenges in standardization due to varied sample preparation, user uncertainty, biological differences, and instrument settings. Diverse user gating strategies further contribute to data variability. Reliable standardization is essential for reproducible results and valid research conclusions.

Imaging-enhanced flow cytometers, like the Attune CytPix, enable real-time sample visualization, allowing users to refine gating strategies and provide clear, documented evidence for research publications. Direct visual confirmation of events reduces data ambiguity and user-dependent variations, ensuring robust, quantitative analyses at the single-cell level.

TITLE: Good Laboratory Practices for Proteomics Workflows

Proteomics is the study of all the proteins in a biological system, aiming to understand their functions and roles. This market size is ever growing field which is expected to grow further with increasing rate of clinical diagnostics and drug discoveries.

Thanks to technological improvements of mass spectrometry, proteomics reached high sensitivity, accuracy and high throughput across multiple conditions with less sample than western blotting and no antibodies needed.

However, with great benefits comes great responsibility. Failed clinical trials may cost additional 0.8-1.4 bn USD per company as only ~30% of candidate drugs advance to phase III trials.

For this reason, implementation of good laboratory practice is essential to avoid incomparable results, long troubleshooting phases, rejected publications and unknown status of the experiment.

Find out more about QC tools and design valuable to implement in proteomics workflow such as reference materials, system suitability test, control charts troubleshooting ideas and more.